Oxidation of Carbon Monoxide and Methane by Pseudomonas methanica
- 1 November 1975
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 91 (1), 79-91
- https://doi.org/10.1099/00221287-91-1-79
Abstract
The oxidation of CO and methane by suspensions and ultrasonic extracts of P. methanica was studied. A continuous assay for the oxidation of CO to CO2 was devised, using O2 and CO2 electrodes in combination. Stoicheiometries of CO-dependent CO2 formation, O2 consumption and NADH oxidation, and the partial stoicheiometries of methane-dependent NADH oxidation, suggest the involvement of a mono-oxygenase in these oxidations. Evidence is presented suggesting methane and CO oxidation are catalyzed by a single enzyme system, distinct, at least in part, from the NADH oxidase present in extracts. Ethanol was able to provide the reductant necessary for CO oxidation by cell suspensions, though the metabolism of ethanol by P. methanica was unlikely to result in substrate-level formation of NADH; the means whereby alcohol oxidation could supply reductant for the mono-oxygenase are discussed.This publication has 7 references indexed in Scilit:
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