A cycloheximide sensitivity factor from yeast required for N-acetylphenylalanylpuromycin formation
- 2 November 1976
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 15 (22), 4760-4764
- https://doi.org/10.1021/bi00667a002
Abstract
A protein (factor P) was isolated from yeast, which was required for sensitivity to cycloheximide of a partially purified polyphenylalanine synthesis system. In the absence of factor P, 10-3 M cycloheximide was required for 50% inhibition of polyphenylalanine synthesis, while, in its presence, 10-6 M gave 50% inhibition. Coincident with cycloheximide sensitivity was an activity required for EF-2 dependent N-acetylphenylalanylpuromycin (N-AcPhePuro) formation. Transfer of N-AcPhe to puromycin from the tRNA bound in the presence of 26 mM MgCl2 required factor P, as well as EF-2. Studies with [rabbit] antibody against EF-2 demonstrated that P factor was not required during the EF-2 translocation step but for some subsequent step.This publication has 4 references indexed in Scilit:
- Structure, Function and Evolution of RibosomesEuropean Journal of Biochemistry, 1976
- Cycloheximide resistance in yeast: A property of the 60s ribosomal subunitBiochemical and Biophysical Research Communications, 1967
- Initiation of polyphenylalanine synthesis by N-acetylphenylalanyl-SRNA.Proceedings of the National Academy of Sciences, 1967
- The behaviour of acetylphenylalanyl soluble ribonucleic acid in polyphenylalanine synthesisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965