Yeast is unable to excise foreign intervening sequences from hybrid gene transcripts.

Abstract

To investigate whether transcripts from foreign split genes are correctly processed in yeast cells 2 hybrid genes were constructed by inserting into the split yeast actin gene an intron-containing fragment from either the Acanthamoeba actin I gene or the duck .alpha.D-globin gene. The hybrid genes were inserted into the autonomously replicating yeast plasmid YRp7, which was then used to transform yeast cells. The yeast but not the foreign intervening sequences were excised from the chimeric transcripts. The recognition of intervening sequences or the splicing mechanism of RNA polymerase II transcripts is apparently not universal.