Taking Advantage of Unspecific Interactions to Produce Highly Active Magnetic Nanoparticle−Antibody Conjugates
- 28 April 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Nano
- Vol. 5 (6), 4521-4528
- https://doi.org/10.1021/nn200019s
Abstract
Several strategies for linking antibodies (Abs) through their Fc region in an oriented manner have been proposed at the present time. By using these strategies, the Fab region of the Ab is available for antigen molecular recognition, leading to a more efficient interaction. Most of these strategies are complex processes optimized mainly for the functionalization of surfaces or microbeads. These methodologies imply though the Ab modification through several steps of purification or the use of expensive immobilized proteins. Besides, the functionalization of magnetic nanoparticles (MNPs) turned out to be much more complex than expected due to the lack of stability of most MNPs at high ionic strength and non-neutral pH values. Therefore, there is still missing an efficient, easy and universal methodology for the immobilization of nonmodified Abs onto MNPs without involving their Fab regions during the immobilization process. Herein, we propose the functionalization of MNPs via a two-steps strategy that takes advantage of the ionic reversible interactions between the Ab and the MNP. These interactions make possible the orientation of the Ab on the MNP surface before being attached in an irreversible way via covalent bonds. Three Abs (Immunoglobulin G class) with very different isoelectric points (against peroxidase, carcinoembryonic antigen, and human chorionic gonadotropin hormone) were used to prove the general applicability of the strategy here proposed and its utility for the development of more bioactive NPs.Keywords
This publication has 61 references indexed in Scilit:
- Functional nanoparticles for molecular imaging guided gene deliveryNano Today, 2010
- Engineered Biocompatible Nanoparticles for in Vivo Imaging ApplicationsJournal of the American Chemical Society, 2010
- Emerging applications of nanomedicine for the diagnosis and treatment of cardiovascular diseasesTrends in Pharmacological Sciences, 2010
- Design and fabrication of magnetic nanoparticles for targeted drug delivery and imagingAdvanced Drug Delivery Reviews, 2009
- Promotion of multipoint covalent immobilization through different regions of genetically modified penicillin G acylase from E. coliProcess Biochemistry, 2009
- Nano-bio-chips for high performance multiplexed protein detection: Determinations of cancer biomarkers in serum and saliva using quantum dot bioconjugate labelsBiosensors and Bioelectronics, 2009
- Magnetic nanoparticles for theragnosticsAdvanced Drug Delivery Reviews, 2009
- Nanoparticle−Target Interactions Parallel Antibody−Protein InteractionsAnalytical Chemistry, 2009
- Epoxy-Amino Groups: A New Tool for Improved Immobilization of Proteins by the Epoxy MethodBiomacromolecules, 2003
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976