The role of a conserved guanosine residue in the hammerhead‐type RNA enzyme
- 2 July 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 267 (1), 150-152
- https://doi.org/10.1016/0014-5793(90)80311-6
Abstract
We have designed a hammerhead-type RNA system which consists of three RNA fagments for normal and modified complexes which contain a non-cleavable substrate with 2'-O-methylcytidine and a guanosine-to-inosine replaced enzyme. Examination of the RNA-cleaving activity and conformational properties of the complexes suggests that the 2-amino group of a conserved guanosine residue in the loop region plays an important role for maintaining both the activity and loop conformation.Keywords
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