Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains
Open Access
- 1 December 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 85 (4), 876-878
- https://doi.org/10.1104/pp.85.4.876
Abstract
Sperm cells were isolated from corn (Zea mays L.) tricellular pollen grains. They were released using a light osmotic chock, and separated from pollen contaminants (especially starch grains) by a Percoll gradient centrifugation. Isolated sperm cells (3 × 106 per milliliter) show a high viability score (90%) as demonstrated with the fluorochromatic reaction. They appeared as spherical cells which lack cell wall and plastids, and can be considered as haploid protoplasts.This publication has 8 references indexed in Scilit:
- Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivumPlant Physiology, 1987
- Isolation of Sperm Cells from the Pollen of Plumbago zeylanicaPlant Physiology, 1986
- Ultrastructural and cytochemical changes of the head components of human spermatids and spermatozoaGamete Research, 1986
- Preferential fertilization in Plumbago : Ultrastructural evidence for gamete-level recognition in an angiospermProceedings of the National Academy of Sciences, 1985
- Elimination of plastids during spermatogenesis and fertilization in the plant kingdomPlasmid, 1980
- Isolation of vegetative and generative nuclei from pollen tubesExperimental Cell Research, 1972
- Evaluation of Pollen Viability by Enzymatically Induced Fluorescence; Intracellular Hydrolysis of Fluorescein DiacetateStain Technology, 1970
- THE ESSENTIAL ROLE OF CALCIUM ION IN POLLEN GERMINATION AND POLLEN TUBE GROWTHAmerican Journal of Botany, 1963