Use of dye-labeled protein as spectrophotometric assay for protein precipitants such as tannin
- 1 November 1985
- journal article
- research article
- Published by Springer Nature in Journal of Chemical Ecology
- Vol. 11 (11), 1535-1544
- https://doi.org/10.1007/bf01012199
Abstract
Bovine serum albumin has been covalently labeled with Remazol brilliant blue R to provide a substrate for a convenient spectrophotometric assay for protein precipitants. The blue protein is especially useful for measuring protein precipitation by vegetable tannins because its absorption maximum is at a wavelength where plant pigments exhibit minimum absorption. Blue BSA has been used to determine, by competition experiments, the relative affinity of various proteins for tannins. A procedure for purifying condensed tannin from commercially available quebracho extract is described.This publication has 28 references indexed in Scilit:
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