DOUBLE ISOTOPE DERIVATIVE ASSAY OF ALDOSTERONE, CORTICOSTERONE, AND CORTISOL
- 1 September 1964
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 42 (9), 1275-1291
- https://doi.org/10.1139/o64-137
Abstract
A method of double isotope derivative assay of aldosterone, corticosterone, and cortisol is described. It involves acetylation with acetic-3H anhydride of high specific activity followed by the purification of the steroids through four paper chromatographies and the formation of a derivative by chromium trioxide oxidation. The method is particularly suited to the quantitative measurement of these steroids in the incubation media of either rat or beef adrenal slices.Keywords
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