Fine structure and evolution of the rat serum albumin gene.
Open Access
- 1 October 1981
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 1 (10), 871-883
- https://doi.org/10.1128/mcb.1.10.871
Abstract
The exons, their boundaries, and approximately half of the intronic deoxyribonucleic acid of the rat serum albumin gene were sequenced. In addition to the 14 exons identified earlier by R-loop analysis, a small exon was detected between the "leader" exon (Z) and exon B. The leader exon encoded the 5'-untranslated portion of albumin messenger ribonucleic acid and the "pre-pro" oligopeptide present on the nascent protein. The sites of initiation and termination of transcription were tentatively identified by comparison of the 5' and 3' gene-flanking sequences with those of other eucaryotic genes. All 28 intron/exon junctions conformed to the "GT-AG rule" (Breathnach et al., Proc. Natl. Acad. Sci. 75:4853-4857, 1978). The three homologous domains of albumin were encoded by three subgenes that consisted of four exons each and evolved by intragenic duplication of a common ancestor. The second and forth exons of each subgene appeared to be the result of an even earlier duplication event. We propose a model for the evolution of this gene that accounts for the observed patterns of exon size and homology.This publication has 42 references indexed in Scilit:
- Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin.Proceedings of the National Academy of Sciences, 1981
- Rat liver pre-proalbumin: complete amino acid sequence of the pre-piece. Analysis of the direct translation product of albumin messenger RNA.Journal of Biological Chemistry, 1977
- Physical properties of chemically acetylated rat liver chromatin.Proceedings of the National Academy of Sciences, 1977
- Biochemical EvolutionAnnual Review of Biochemistry, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Structural origins of mammalian albumin.1976
- Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.The Journal of cell biology, 1975
- Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structuresJournal of Molecular Biology, 1975
- Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.Proceedings of the National Academy of Sciences, 1975
- Sites of Binding of Copper(II) Ion by Peptide (1–24) of Bovine Serum AlbuminJournal of Biological Chemistry, 1968