CHEMICAL DETERMINATION OF ALDOSTERONE IN URINE
- 1 October 1959
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 19 (10), 1302-1311
- https://doi.org/10.1210/jcem-19-10-1302
Abstract
A method for the determination of aldosterone in urine is presented, which includes the following steps: acid hydrolysis and extraction, column chromatography on silica gel, 2 paper chromatographic systems, acetylation, a third paper chromatographic system, and fluorometry directly on the paper using alkaline fluorescence. Data are presented on precision, recovery, duration of acid hydrolysis, acid versus enzymatic hydrolysis, stability of urine samples and normal values.Keywords
This publication has 4 references indexed in Scilit:
- ISOLATION OF A STEROID-LIKE SUBSTANCE FROM URINE AND CITROUS FRUITSCanadian Journal of Biochemistry and Physiology, 1958
- A NEW METHOD FOR THE COLORIMETRIC DETERMINATION OF ALDOSTERONE IN URINEActa Endocrinologica, 1957
- FLUORESCENCE METHOD FOR THE MICROANALYSIS OF DELTA-4-3-OXO STEROIDS ON PAPER CHROMATOGRAMS1957
- ALDOSTERONE EXCRETION IN HEALTHY PERSONS*Journal of Clinical Endocrinology & Metabolism, 1956