Molecular cloning of the region of the terminus of Escherichia coli K-12 DNA replication
- 1 February 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 153 (2), 604-609
- https://doi.org/10.1128/jb.153.2.604-609.1983
Abstract
A series of plasmids were isolated by ligation of defined restriction fragments to plasmid pBR325 or by screening of a cosmid bank by in situ colony hybridization. Together with 1 previously isolated plasmid, they spanned 86% of the 30.5- to 34-min region of the genetic map of E. coli K-12. Physical analysis of these plasmids and hybridizations to Southern blots confirmed the endonuclease map of this region, with the exception of a 9.3-kilobase pair inversion.This publication has 25 references indexed in Scilit:
- Relationships between the physical and genetic maps of a 470 × 103 base-pair region around the terminus of Escherichia coli K12 DNA replicationJournal of Molecular Biology, 1982
- Physical map of a 470 × 103 base-pair region flanking the terminus of DNA replication in the Escherichia coli K12 genomeJournal of Molecular Biology, 1982
- Distribution of IS5 in bacteriaGene, 1981
- Distribution of the insertion sequence IS1 in Gram-negative bacteriaNature, 1981
- A small cosmid for efficient cloning of large DNA fragmentsGene, 1980
- Plasmid screening at high colony densityGene, 1980
- Evidence for a fixed termination site of chromosome replication in Escherichia coli K12Journal of Molecular Biology, 1977
- Rat Insulin Genes: Construction of Plasmids Containing the Coding SequencesScience, 1977
- Deletions and insertions in the immunity region of coliphage lambda: Revised measurement of the promoter-startpoint distanceVirology, 1974
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966