THE SEPARATION OF FERRITIN AND HAEMOSIDERIN FOR STUDIES IN THE METABOLISM OF IRON
- 1 April 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 95 (1), 282-288
- https://doi.org/10.1042/bj0950282
Abstract
Ferritin and hemosiderin in diluted tissue cannot be quantitatively separated by centrifugation unless the pH is brought below 6. Tnese Fe-storage substances can be separated from one another and from hemoglobin by ion-exchange chromatography on CM-cellulose, when good recoveries of total tissue iron are obtained. The ferritin fraction has been identified by its solubility, its appearance under the electron microscope, crystallization with Cd2+ and the fact that it corresponds quantitatively to the heat-stable portion of the tissue non-hem Fe.Keywords
This publication has 22 references indexed in Scilit:
- Studies on intermediary iron metabolism. XII. Measurement of the iron derived from water soluble and water insoluble non-haem compounds (ferritin and haemosiderin iron) in liver and spleen.1958
- The Effects of Acute Protein Depletion on the Distribution of Radioiron in Rat TissuesBlood, 1958
- A STUDY OF HEMOSIDEROSIS WITH THE AID OF ELECTRON MICROSCOPYThe Journal of Experimental Medicine, 1957
- Hemosiderin. Isolation from Horse Spleen and Characterization.Experimental Biology and Medicine, 1957
- Iron metabolism in the laying henBiochemical Journal, 1954
- An electron microscopic study of ferritinBiochimica et Biophysica Acta, 1954
- [Physiology and pathology of stored iron. 1. A method for quantitative determination of ferritin].1954
- A QUANTITATIVE FRACTIONATION OF TISSUE FERRITIN AND HEMOSIDERINJournal of Biological Chemistry, 1953
- THE RELATIONSHIP BETWEEN FERRITIN AND HEMOSIDERIN IN RABBITS AND MANJournal of Biological Chemistry, 1953
- Uptake and Storage of Radioactive Iron by Mouse LiverAmerican Journal of Physiology-Legacy Content, 1953