THE SEPARATION OF FERRITIN AND HAEMOSIDERIN FOR STUDIES IN THE METABOLISM OF IRON
- 1 April 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 95 (1), 282-288
- https://doi.org/10.1042/bj0950282
Abstract
Ferritin and hemosiderin in diluted tissue cannot be quantitatively separated by centrifugation unless the pH is brought below 6. Tnese Fe-storage substances can be separated from one another and from hemoglobin by ion-exchange chromatography on CM-cellulose, when good recoveries of total tissue iron are obtained. The ferritin fraction has been identified by its solubility, its appearance under the electron microscope, crystallization with Cd2+ and the fact that it corresponds quantitatively to the heat-stable portion of the tissue non-hem Fe.This publication has 22 references indexed in Scilit:
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