High‐Affinity, Sodium‐Dependent Γ‐Aminobutyric Acid Uptake by Slices of Rat Ovary
- 1 February 1983
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 40 (2), 582-584
- https://doi.org/10.1111/j.1471-4159.1983.tb11322.x
Abstract
The high concentration of gamma-aminobutyric acid (GABA) recently demonstrated in rat ovary prompted us to examine the capacity of ovarian slices to take up [3H]GABA. Active uptake, dependent on temperature and sodium concentration, was observed and a kinetic constant (Km) of 1.0 microM found for the uptake process. Ouabain (100 microM) reduced the rate of accumulation of [3H]GABA. Uptake was inhibited only partially by 100 microM d,l-nipecotic acid, but more strongly by 100 microM beta-alanine. These results suggest that the uptake system in ovary possesses properties similar to those of high-affinity GABA transport systems in the brain.Keywords
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