THE AMINO ACID COMPOSITION OF CRYSTALLINE PEPSIN
Open Access
- 19 January 1959
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 42 (3), 553-561
- https://doi.org/10.1085/jgp.42.3.553
Abstract
The amino acid composition of twice recrystallized pepsin (Worthington Biochemical Corporation) has been determined chromatographically on columns of Amberlite IR 120 resin. The results of the analyses obtained on four different preparations indicate a close agreement in their amino acid composition. Pepsin is unique in that it has a great predominance of acidic amino acids over basic ones. Moreover, all the preparations contain a small and constant amount of hydroxyproline, corresponding to about 0.1 residue per molecule.Keywords
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