An Exopectate Lyase of Butyrivibrio fibrisolvens from the Bovine Rumen
- 1 November 1982
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 128 (11), 2661-2665
- https://doi.org/10.1099/00221287-128-11-2661
Abstract
An extracellular pectinolytic enzyme produced by B. fibrisolvens isolated from the bovine rumen was studied. The enzyme had a pH optimum of 8.0-8.5 was stimulated by Ca2+ and inhibited by EDTA. The products of pectinolysis had an absorption peak at 235 nm and reacted with thiobarbituric acid, indicating a lyase type of action. The enzyme cleaved the substrates terminally from the reducing end; action on poly- and oligogalacturonates resulted in the formation of an unsaturated trigalacturonate. The enzyme was classified as an exopectate lyase (EC 4.2.2.2). A pectinesterase was also produced by B. fibrisolvens but polygalacturonase was not detected.This publication has 7 references indexed in Scilit:
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