VH gene family utilization is regulated by a locus outside of the VH region.
Open Access
- 1 April 1988
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 167 (4), 1499-1504
- https://doi.org/10.1084/jem.167.4.1499
Abstract
We have used the RNA colony blot method to examine VH usage in colonies derived from primary splenic B cells. We found that there are strain-specific differences in the pattern of VH usage. Using parental F1, congenic, and recombinant inbred strains we demonstrate that the genetic element that causes the observed phenotype is: (a) stably expressed; (b) not due to maternal influence; (c) not due to dominate diffusible factors; (d) not linked to cloning efficiency; and (e) outside the Igh locus.Keywords
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