Somatic mutation creates diversity in the major group of mouse immunoglobulin kappa light chains.
Open Access
- 1 February 1984
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 159 (2), 417-435
- https://doi.org/10.1084/jem.159.2.417
Abstract
Using a cloned cDNA of a mouse immunoglobulin kappa light chain synthesized in a myeloma MOPC321 (V kappa-21 subgroup C) as a probe we could detect 13 germ line V kappa gene segments. 11 of these were isolated. Using a set of overlapping cloned segments, we showed that nine of these germ line V kappa genes are arranged in two linkage clusters and that they all have the same transcriptional orientation (11, 12, 22). These two clusters occupy 90 and 30 kb of chromosomal DNA and contain six and three V kappa's, respectively. We determined the complete nucleotide sequences of five germ line V kappa's and showed that three of them encode the prototype sequence of V kappa-21 subgroups B, C, and E. None of these five germ line V kappa's encodes the variant amino acid sequences of known V kappa-21 subgroups. We thus conclude that, as in the lambda 1 light chains, the variant V regions are encoded by gene segments derived by a few somatic mutations from the corresponding germ line DNA. Such somatic mutations are not restricted to sequences encoding the hypervariable regions: they also occur in sequences encoding framework regions.This publication has 53 references indexed in Scilit:
- Somatic mutation of immunoglobulin light-chain variable-region genesCell, 1981
- Expression of kappa chains of the Vκ21 group in Mus musculus and related speciesMolecular Immunology, 1981
- Ordering of mouse immunoglobulin heavy chain genes by molecular cloningNature, 1980
- A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing eventsNature, 1980
- Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segmentsNature, 1979
- Multiplicity of germline genes specifying a group of related mouse κ chains with implications for the generation of immunoglobulin diversityNature, 1978
- Genetic studies of the lac repressorJournal of Molecular Biology, 1978
- Cloned MPC 11 myeloma cells express two kappa genes: a gene for a complete light chain and a gene for a constant region polypeptideCell, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Variability in the Lambda Light Chain Sequences of Mouse AntibodyNature, 1970