Oligosaccharide Structural Studies by On-Line HPLC-MS using Fast Atom Bombardment Ionization

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Abstract
A moving polyimide belt was used as an HPLC-MS interface for the purification and structural studies of oligosaccharides. The samples, desorbed by FAB in the absence of added liquid matrix, exhibited the following characteristics: a) good signal to noise ratio at low mass; b) a variation in the degree of fragmentation and relative sensitivity with changes in belt speed; c) a diminished sensitivity to signal suppression by contaminants when contrasted to FAB matrix; and d) fragment ions which can provide useful structural information. The moving belt HPLC-MS interface was tested with a series of known standards and a sample of high mannose oligosaccharides isolated from animal urines.