Triple resonance HNCCCH experiments for correlating exchangeable and nonexchangeable cytidine and uridine base protons in RNA
- 1 December 1995
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 6 (4), 427-432
- https://doi.org/10.1007/bf00197641
Abstract
A set of triple resonance experiments is presented, providing through-bond H2N/HN to H6 connectivities in uridines and cytidines in 13C-/15N-labeled RNAs. These connectivities provide an important link between the sequential assignment pathways for the exchangeable and nonexchangeable proton resonances in nucleic acids. Both 2D and pseudo-3D HNCCCH experiments were applied to a 30-nucleotide lead-dependent ribozyme, known as the leadzyme. The HN to H6 connectivities for three uridines in the leadzyme were identified from one 2D H(NCCC)H experiment, and the H2N to H6 connectivities were identified for seven of the eight cytidines from the combination of a 2D H(NCCC)H and a pseudo-3D H(NCC)CH experiment.This publication has 40 references indexed in Scilit:
- Improved measurement of13C,31P J coupling constants in isotopically labeled RNAFEBS Letters, 1995
- NMR Pulse Schemes for the Sequence-Specific Assignment of Arginine Guanidino 15N and 1H Chemical Shifts in ProteinsJournal of the American Chemical Society, 1995
- Three-Dimensional Triple-Resonance 1H, 13C, 31P Experiment: Sequential Through-Bond Correlation of Ribose Protons and Intervening Phosphorus along the RNA Oligonucleotide BackboneJournal of the American Chemical Society, 1994
- An Alternative Triple-Resonance Method for the Through-Bond Correlation of Intranucleotide H1' and H8 NMR Signals of Purine Nucleotides. Application to a DNA Dodecamer with Fully 13C/15N-Labeled Deoxyadenosine ResiduesJournal of the American Chemical Society, 1994
- Correlation of Adenine H2/H8 Resonances in Uniformly 13C Labeled RNAs by 2D HCCH-TOCSY: A New Tool for 1H AssignmentJournal of the American Chemical Society, 1994
- Two-dimensional triple-resonance HCNCH experiment for direct correlation of ribose H1' and base H8, H6 protons in 13C,15N-labeled RNA oligonucleotidesJournal of the American Chemical Society, 1993
- Unambiguous resonance assignments in carbon-13, nitrogen-15-labeled nucleic acids by 3D triple-resonance NMRJournal of the American Chemical Society, 1993
- An Efficient Procedure for Assignment of the Proton, Carbon and Nitrogen Resonances in 13C/15N Labeled Nucleic AcidsJournal of Molecular Biology, 1993
- Simple procedure for resonance assignment of the sugar protons in carbon-13 labeled RNAsJournal of the American Chemical Society, 1992
- Two-dimensional hetero-TOCSY-NOESY. Correlation of phosphorus-31 resonances with anomeric and aromatic proton resonances in RNAJournal of the American Chemical Society, 1992