Construction of a binding site for human immunodeficiency virus type 1 gp120 in rat CD4.
Open Access
- 1 January 1992
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 175 (1), 301-304
- https://doi.org/10.1084/jem.175.1.301
Abstract
The human immunodeficiency virus (HIV-1) infects T lymphocytes via an interaction between the virus envelope glycoprotein gp120 and the CD4 antigen of T helper cells. Previous studies demonstrated that mutations in various regions of CD4 domain 1 lead to the loss of gp120 binding. In the present study the gp120 binding site was constructed in rat CD4 by replacing rat with human CD4 sequence. A series of mutants was constructed the best of which bound gp120 with an affinity only twofold less than that of human CD4. The data indicate that the gp120 binding site of human CD4 is constituted by residues 33-58 of domain 1.Keywords
This publication has 17 references indexed in Scilit:
- A highly selected panel of anti-CD4 antibodies fails to induce anti-idiotypic antisera mediating human immunodeficiency virus neutralizationEuropean Journal of Immunology, 1991
- Crystal structure of an HIV-binding recombinant fragment of human CD4Nature, 1990
- Atomic structure of a fragment of human CD4 containing two immunoglobulin-like domainsNature, 1990
- DIFFERENCES IN AFFINITY OF ANTI-CD4 MONOCLONAL-ANTIBODIES PREDICT THEIR EFFECTS ON SYNCYTIUM INDUCTION BY HUMAN-IMMUNODEFICIENCY-VIRUS1990
- Substitution of murine for human CD4 residues identifies amino acids critical for HIV-gp120 bindingNature, 1988
- Genetic analysis of monoclonal antibody and HIV binding sites on the human lymphocyte antigen CD4Cell, 1988
- Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.Proceedings of the National Academy of Sciences, 1987
- Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: evidence for derivation from a structure with four immunoglobulin-related domains.Proceedings of the National Academy of Sciences, 1987
- The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brainCell, 1986
- Binding of HTLV-III/LAV to T4 + T Cells by a Complex of the 110K Viral Protein and the T4 MoleculeScience, 1986