Methotrexate: Studies on cellular metabolism. II—Effects on mitochondrial oxidative metabolism and ion transport
- 1 April 1989
- journal article
- research article
- Published by Wiley in Cell Biochemistry and Function
- Vol. 7 (2), 129-134
- https://doi.org/10.1002/cbf.290070208
Abstract
Effects of methotrexate (MTX) on mitochondrial oxidative metabolism and ion transport were studied. MTX decreases the membrane potential (Δψ) upon energization of the mitochondrial membrane by NAD+-linked substrates and decreases the amplitude and velocity of swelling induced by glutamate and α-ketoglutarate. MTX also has an inhibitory effect on the activities of the oxidation enzymes of NAD+-linked substrates without interfering with the oxidation systems of FAD-linked substrates. The effects of MTX could be interpreted as a consequence of a decrease in the ionic conductivity of the mitochondrial inner membrane.Keywords
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