In in vitro production and cloning of the P cell, a bone marrow-derived null cell that expresses H-2 and Ia-antigens, has mast cell-like granules, and is regulated by a factor released by activated T cells.
- 1 February 1981
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 126 (2), 452-458
- https://doi.org/10.4049/jimmunol.126.2.452
Abstract
A technique, based on selective culture conditions, is described for the preparation from mouse spleen of a pure population of Ig-negative, Thy.1-negative and Lyt.1- and Lyt.2-negative nonadherent cells, termed persisting (P) cells. Antigens coded for by the H2-K, H2-D, and I regions were readily detectable on the surface of P cells. P cells grew for long periods in vitro provided that a factor produced by activated T cells, but distinct from T cell growth factor, was present. It was demonstrated that P cells were bone marrow derived. When P cells were examined in suspension, a variable number of fine cytoplasmic projections could be seen waving from the cell, often at 1 pole. The nuclei varied in shape, being round, in general, but with bilobed and irregular forms occurring, and the cytoplasm contained large numbers of characteristic metachromatic granules. P cells had readily detectable Fc receptors but did not phagocytose latex particles or antibody-coated erythrocytes. P cells formed colonies in soft agar in the presence of growth factors, and cloned lines could be propagated from these colonies. The relationship of P cells to mast cells and Ia-positive accessory cells and their possible role in T cell activation is discussed.This publication has 20 references indexed in Scilit:
- Lymphoid dendritic cells are potent stimulators of the primary mixed leukocyte reaction in mice.Proceedings of the National Academy of Sciences, 1978
- Cellular and genetic control of antibody responses in vitro. III. Immune response gene regulation of accessory cell functionThe Journal of Experimental Medicine, 1978
- On the thymus in the differentiation of "H-2 self-recognition" by T cells: evidence for dual recognition?The Journal of Experimental Medicine, 1978
- CELLULAR AND GENETIC-CONTROL OF ANTIBODY-RESPONSES INVITRO .4. EXPRESSION OF IA ANTIGENS ON ACCESSORY CELLS REQUIRED FOR RESPONSES TO SOLUBLE-ANTIGENS INCLUDING A RESPONSE UNDER IR GENE CONTROL1978
- Enrichment of specific suppressor T cells and characterization of their surface markers.The Journal of Experimental Medicine, 1977
- Histocompatibility linked immune responsiveness and restrictions imposed on sensitized lymphocytes.The Journal of Experimental Medicine, 1977
- Functional Specificity of Thymus-Dependent LymphocytesScience, 1977
- Purification and properties of colony-stimulating factor from mouse lung-conditioned medium.Journal of Biological Chemistry, 1977
- Selective in Vitro Growth of T Lymphocytes from Normal Human Bone MarrowsScience, 1976
- Role of macrophages in the generation of T helper cells. IV. Nature of genetically related factor derived from macrophages incubated with soluble antigensEuropean Journal of Immunology, 1976