Autoradiographic Localization of Peripheral Benzodiazepine, Dihydroalprenolol and Arginine Vasopressin Binding Sites in the Pituitaries of Control, Stalk Transected and Brattleboro Rats
- 1 January 1986
- journal article
- research article
- Published by S. Karger AG in Neuroendocrinology
- Vol. 44 (1), 76-83
- https://doi.org/10.1159/000124625
Abstract
The autoradiographic distribution of [3H]arginine vasopressin, [3H]spiperone, [3H]GABA, [3H]dihydroalprenolol and the peripheral-type benzodiazepine ligand [3H]Ro5-4864 were examined in the rat pituitary before and after pituitary stalk transection. Stalk transection produced dramatic changes in the cellular architecture of the pars nervosa. Glial fibrillary acidic protein, an astrocyte marker reported in pituicytes, increased after stalk transection, whereas neurofilament, a marker for neuronal innervation, was lost. These structural changes demonstrated a successful stalk transection, permitting interpretation of changes in the densities of several [3H]-ligands over the three lobes. [3H]Ro5-4864 binding was markedly increased, suggesting that this site was located on the pituicytes. Conversely [3H]spiperone and [3H]arginine vasopressin binding density over the pars nervosa decreased. In the mutant diabetes insipidus rat (Brattleboro), which lacks pituitary vasopressin, [3H]arginine vasopressin binding was undetectable in the pars nervosa. [3H]dihydroalprenolol and [3H]GABA binding sites were unchanged by the lesion. These results are discussed in terms of the occurrence of functional acceptors on pituicytes and their possible role in neurohydrophyseal secretions.Keywords
This publication has 12 references indexed in Scilit:
- Studies of the Nature of the Interaction between Vasopressin and Corticotropin-Releasing Factor on Adrenocorticotropin Release in the Rat*Endocrinology, 1984
- Single base deletion in the vasopressin gene is the cause of diabetes insipidus in Brattleboro ratsNature, 1984
- Arginine-vasopressin binding sites in rat brain: A quantitative autoradiographic studyNeuroscience Letters, 1984
- [3H]Ro5-4864 benzodiazepine binding in the kainate lesioned striatum and Huntington's diseased basal gangliaBrain Research, 1982
- Immunoreactive glial fibrillary acidic protein in pituicytes of the rat neurohypophysisBrain Research, 1982
- CHARACTERIZATION OF PERIPHERAL-TYPE BENZODIAZEPINE BINDING-SITES IN BRAIN USING (H-3)-LABELED RO-5-48641982
- Radial glia in the developing mouse cerebral cortex and hippocampusBrain Structure and Function, 1981
- Identification of the pituicytes as astroglial cells by indirect immunofluorescence-staining for the glial fibrillary acidic proteinBrain Research, 1981
- Specific neuronal localization by immunofluorescence of 10 nm filament polypeptidesJournal of Neurocytology, 1980
- Biochemical Identification of the β-Adrenoceptor and Evidence for the Involvement of an Adenosine 3′,5′-Monophosphate System in the β-Adrenergically Induced Release of a-Melanocyte-Stimulating Hormone in the Intermediate Lobe of the Rat Pituitary GlandEndocrinology, 1980