Recombination between two mouset-haplotypes (tw12tfandtLub-1): segregation of lethal factors relative to centromere and tufted (tf) locus

Abstract

SUMMARY: Recombination between two mouset–haplotypes,t^w12tfandt^Lub-1, was investigated by screening the tailless progeny of the cross ♀t^w12tf/t^Lub-1+ × ♂Ttf/+tffor the segregation of tufted phenotype,t^w12andt^Lub-1lethal factors, and metacentric chromosome (sincet^Lub-lhaplotype is associated with a Robertsonian fusion involving chromosomes 4 and 17). The results give a 17% estimate of the recombination frequency between centromere andtf, witht^Lub-llethal factor mapping about two-thirds of the distance from centromere totfand thet^w12lethal factor behaving as if closely linked totf. This further extends the findings of Silver & Artzt (1981) and of Artzt, McCormick & Bennett (1982), and shows that twot–haplotypes with quite independent laboratory histories recombine at a normal level, supporting the notion that allt–haplotypes basically share the same structure.