Amino acid sequence of honeybee prepromelittin synthesized in vitro.
- 1 February 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (2), 701-704
- https://doi.org/10.1073/pnas.75.2.701
Abstract
Translation of melittin mRNA from queen bee venom glands in a cell-free system from wheat germ yielded prepromelittin. Sequence analysis of the labeled in vitro product was performed by automatic Edman degradation of the intact polypeptide and by analysis of some of its proteolytic fragments. Prepomelittin was composed of 70 amino acids, 2 of which were not identified. The sequence of melittin is located in the COOH-terminal third of the polypeptide chain (residues 44-69). Prepromelittin starts with a very hydrophobic pre-region, probably 21 residues long, followed by a pro-part of unusual sequence, containing only alanine proline and acidic residues. At least 3 post-translational reactions are required to convert prepromelittin to melittin.Keywords
This publication has 22 references indexed in Scilit:
- Isolation, crystallization, and primary amino acid sequence of human platelet factor 4.Journal of Biological Chemistry, 1977
- Cell-free synthesis of fish preproinsulin, and processing by heterologous mammalian microsomal membranes.Proceedings of the National Academy of Sciences, 1977
- Rat liver preproalbumin: in vitro synthesis and partial amino acid sequence.Proceedings of the National Academy of Sciences, 1977
- Translation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide.Proceedings of the National Academy of Sciences, 1977
- Amino acid sequence of the NH2-terminal extra piece segments of the precursors of mouse immunoglobulin lambda1-type and kappa-type light chains.Proceedings of the National Academy of Sciences, 1977
- Partial amino acid sequence of rat pre-prolactinBiochemical Journal, 1977
- Partial amino acid sequence of human placental lactogen precursor and its mature hormone form produced by membrane-associated enzyme activityBiochemical and Biophysical Research Communications, 1977
- Characterization of promelittin messenger RNA from the venom gland of young queen beesJournal of Molecular Biology, 1976
- Insect protein synthesis in frog cells: The translation of honey bee promelittin messenger RNA in Xenopus oocytesJournal of Molecular Biology, 1974
- A Possible Precursor of Immunoglobulin Light ChainsNature New Biology, 1972