Isolation and Identification of Testosterone in Systemic Blood from Normal Human Male Adults.

Abstract

By criteria of paper chromatog-raphy in 2 systems, formation of an acetate derivative, UV and infrared spectrophotometry, testosterone has been isolated and identified in systemic blood of normal male subjects given human chorionic gonado-tropin intramuscularly. Plasma testosterone could not be found in normal male subjects not stimulated with human chorionic gonadotropin.