An upper limit to the number of sodium channels in frog skin epithelium
- 1 February 1973
- journal article
- Published by Wiley in The Journal of Physiology
- Vol. 228 (3), 681-692
- https://doi.org/10.1113/jphysiol.1973.sp010106
Abstract
1. Binding of [(14)C]amiloride to the mucosal surface of frog skin epithelium has been measured in the presence and absence of an excess of unlabelled amiloride. Simultaneous records were obtained of the sodium transport through the skin as indicated by the short circuit current.2. The number of binding sites with affinities for amiloride of 5 x 10(7) l./mole was around 400/mum(2).3. The relation between the binding sites and sodium channels is discussed.4. Making certain assumptions it is concluded that when frog skin epithelium is bathed in Ringer solution each sodium channel can handle 8000 sodium ions/sec.Keywords
This publication has 15 references indexed in Scilit:
- The Low‐Frequency Electrical Impedance of the Isolated Frog SkinActa Physiologica Scandinavica, 1971
- The binding of tetrodotoxin to nerve membranesThe Journal of Physiology, 1971
- The effects of saponins on the transporting epithelium of isolated frog skin.1970
- Changes in sodium pool and kinetics of sodium transport in frog skin produced by amilorideBritish Journal of Pharmacology, 1970
- Effects of amiloride on active sodium transport by the isolated frog skin: evidence concerning site of actionBritish Journal of Pharmacology, 1970
- Effect of Amiloride on sodium transport in frog skinPflügers Archiv - European Journal of Physiology, 1970
- Effect of amiloride on sodium transport of frog skinPflügers Archiv - European Journal of Physiology, 1970
- Transport and distribution of sodium across frog skinThe Journal of Physiology, 1967
- An upper limit to the number of sodium channels in nerve membrane?The Journal of Physiology, 1967
- The drug-receptor complexJournal of Pharmacy and Pharmacology, 1966