Plasma concentrations of 1-deamino-8-d-arginine vasopressin after intragastric administration in the rat

Abstract
A sensitive and specific radioimmunoassay for 1-deamino-8-D-arginine-vasopressin (DDAVP) was developed. Plasma concentrations of DDAVP after intragastric administration were determined in non-fasted rats and a dose-related increase in plasma levels was obtained. The immunoidentity of plasma DDAVP and standard was established by high performance liquid chromatography (HPLC). Correlation of biological and immunological responses of DDAVP was assessed using the antidiuretic effect in the Brattleboro (diabetes insipidus) rat.