Micro Determination of Cysteine plus Cystine in Proteins.
- 1 January 1961
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 15 (4), 853-855
- https://doi.org/10.3891/acta.chem.scand.15-0853
Abstract
A method is described for microdetermination of the total amount of cysteine and cystine in proteins. By means of hydrazinolysis the cysteine and cystine sulphur is transformed to hydrogen sulphide which is subsequently measured colorimetrically as bismuth sulphide. Quantities of 20[long dash]200 ug cysteine plus cystine can be determined. Neither methionine nor ammonium sulphate interfere with the reaction.This publication has 1 reference indexed in Scilit:
- Amino acid content of leguminous proteins as affected by genetic and nutritional factors IIIArchiv für Mikrobiologie, 1959