Mutational analysis of the tobacco mosaic virus 5′-leader for altered ability to enhance translation
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (3), 883-893
- https://doi.org/10.1093/nar/16.3.883
Abstract
Mutational analysis of the 5''-untranslated leader sequence (.OMEGA.) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with .OMEGA.. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alterations in .OMEGA.''s ability to enhance expression of .beta.-glucuronidase (GUS) mRNA in tobacco mesophyll protoplasts and Escherichia coli or chloramphenicol acetyltransferase (CAT) mRNA in Xenopus laevis oocytes. Alteration of an eight base subsequence required for the binding of a second ribosome resulted in the loss of translational enhancement in X. laevis oocytes but not in protoplasts. Substantial increases in enhancement were observed for several of the mutants in E. coli.This publication has 23 references indexed in Scilit:
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