Granulocyte colony‐stimulating factor down‐regulates the surface expression of the human leucocyte adhesion molecule‐1 on human neutrophils in vitro and in vivo
- 1 August 1993
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 84 (4), 574-580
- https://doi.org/10.1111/j.1365-2141.1993.tb03130.x
Abstract
The leucocyte adhesion molecule-1 (LAM-1) is the human homologue of the murine peripheral lymph node homing receptor, MEL-14, and might play a crucial role in neutrophil localization at inflammatory sites. We have reported previously that recombinant human granulocyte colony-stimulating factor (rhG-CSF) stimulates or enhances several neutrophil functions in vivo, as well as in vitro. To further explore the possible role of G-CSF in inflammation we studied the effect of rhG-CSF on the surface expression of LAM-1 on human neutrophils, both in vitro and in vivo. The expression of LAM-1 by human neutrophils was investigated by indirect immunofluorescence using flow cytometry and monoclonal antibodies anti-Leu-8 and TQ1. A whole blood analysis was performed to minimize in vitro manipulation. Most circulating human neutrophils expressed LAM-1 on the cell surface. Brief exposure of neutrophils to rhG-CSF in vitro decreased the surface expression of LAM-1. rhG-CSF down-regulated neutrophil LAM-1 expression in a time- and dose-dependent manner. Neutrophils from healthy volunteers and from patients who were receiving rhG-CSF exhibited a decreased expression of LAM-1 after rhG-CSF administration, and the expression thereafter returned or overshot the pretreatment level after stopping rhG-CSF administration. These findings indicate that rhG-CSF down-regulates the surface expression of LAM-1 on human neutrophils in vivo, as well as in vitro, and G-CSF might participate in neutrophil-endothelial cell interaction in inflamed tissue.Keywords
This publication has 27 references indexed in Scilit:
- Clinicopathologic and therapeutic aspects of angioimmunoblastic lymphadenopathy‐related lesionsCancer, 1992
- Leukocyte-endothelial cell recognition: Three (or more) steps to specificity and diversityCell, 1991
- Adhesion receptors of the immune systemNature, 1990
- Leukocyte Adhesion Molecules Deficiency: Its Structural Basis, Pathophysiology and Implications for Modulating the Inflammatory ResponseImmunological Reviews, 1990
- Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptorNature, 1989
- Endothelial Leukocyte Adhesion Molecule 1: an Inducible Receptor for Neutrophils Related to Complement Regulatory Proteins and LectinsScience, 1989
- Recognition of an endothelial determinant for CD 18-dependent human neutrophil adherence and transendothelial migration.JCI Insight, 1988
- Neutrophil adherence to human endothelium in vitro occurs by CDw18 (Mo1, MAC-1/LFA-1/GP 150,95) glycoprotein-dependent and -independent mechanisms.JCI Insight, 1988
- Recombinant human granulocyte colony-stimulating factor enhances superoxide release in human granulocytes stimulated by the chemotactic peptideBiochemical and Biophysical Research Communications, 1987
- Recombinant Human Granulocyte Colony-Stimulating Factor: Effects on Normal and Leukemic Myeloid CellsScience, 1986