THE NATURE OF ADRENALINE AND NORADRENALINE IN NORMAL HUMAN URINE*
- 1 February 1956
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 16 (2), 216-221
- https://doi.org/10.1210/jcem-16-2-216
Abstract
Studies were conducted to determine the nature of epinephrine (A) and norepinephrine (NA) present in normal human urine. In addition to acid hydrolysis, the urine was incubated with [beta]-glucuronidase and with Mylase P as a source of phenolsulfatase. With acid hydrolysis, the highest values of NA were obtained after 5 minutes of boiling at pH 1.5. Definite loss of NA and A activity was seen after boiling for periods of 15 to 30 minutes. NA is conjugated to the extent of 60%, whereas A is in all likelihood free. The conjugate of NA in normal urine appears to be the glucuronide and not the sulfate.Keywords
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