Flow cytometric competitive binding assay for determination of actinomycin-D concentrations
Open Access
- 1 January 1990
- Vol. 11 (2), 311-313
- https://doi.org/10.1002/cyto.990110213
Abstract
A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycin-D for common binding sites on DNA coated 10 μm diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the presence of a constant amount of mithramycin, the microsphere fluorescence is inversely proportional to actinomycin-D concentration.Keywords
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