Fe59 Assessment of Erythropoiesis in Mice Following Injections of Anemic Rabbit Plasma Extracts.

Abstract

These studies on male Swiss mice (Hauschka ICR) describe (1) the effectiveness of an erythropoietic factor in deproteinized plasma extract from rabbits made anemic with phenylhydrazine in producing erythropoietic stimulation as judged by Fe59 uptake in red cells, and (2) the effect of hypertransfusion of whole blood on Fe59 uptake. The studies demonstrated the erythropoietic stimulating property of a deproteinized plasma extract (Lot 1) from anemic rabbits. However, plasma extracts from anemic rabbits previously tested in normal and hypophysectomized rats, were more effective with a smaller dose on a per gram body weight basis inasmuch as a dose 6 times greater to the mouse gave an erythropoietic response of lesser magnitude. Moreover, induced plethora affected the bone marrow''s output of red cells, and this response lends support to the concept that Fe59 uptake in the mouse is a valid representation of RBC production.