5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.
- 1 December 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (12), 5323-5327
- https://doi.org/10.1073/pnas.74.12.5323
Abstract
The region of the SV-40 genome complementary to the 5'' end of the most abundant poly(A)-containing 19S and 16S mRNA was mapped by hybridization of double-labeled RNA ([3H]methyl group and [14C]uridine) to specific DNA fragments. Chemical identification of methylated residues indicated that a common leader sequence adjacent to the 5'' terminus of both 19S and 16S mRNA is transcribed from DNA sequences located between 0.67-0.76 map units. The estimated size of this leader RNA, which does not code for any known viral protein, is 170-200 nucleotides. Sequences complementary to the leader region and coding portion of 16S mRNA are apparently located in separate parts of the SV-40 genome.This publication has 15 references indexed in Scilit:
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