Monoclonal antibodies reacting with murine teratocarcinoma cells.
- 1 January 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (1), 377-380
- https://doi.org/10.1073/pnas.76.1.377
Abstract
Monoclonal antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a rat immunized with the C3H mouse teratocarcinoma C86.cntdot.S1. After the fusion 2 clones were chosen for further analysis. Clone 3C4-10 produced an antibody recognizing an antigen with a distribution restricted to teratocarcinoma cell lines, an endoderm cell line and a neuroblastoma. Clone 4A1-9 produced an antibody that reacted with all cultured murine cells tested and adult brain. Neither antibody reacted with preimplantation embryos. The 3C4-10 antibody recognized an antigen associated with proteins. The apparent MW of the 3C4-10 antigen was greater than 100,000.Keywords
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