AN IMPROVED PROCEDURE FOR THE QUANTITATIVE ESTIMATION OF URINARY PREGNANEDIOL

Abstract

An improved procedure for the estimation of urinary pregnanediol is described, with analyses showing recoveries obtained at the different stages. The principle sources of loss were found to be in the initial step of hydrolysis and extraction, where a recovery of 80-85% may be expected, and in the precipitation from acetone -NaOH, where a relatively constant loss of 0.08 mg. occurs. No uniform excretion of pregnanediol was found to occur in relation to volume of urine passed, or to the time of day during which the urine was collected.