Centromeric DNA of Kluyveromyces lactis
- 1 December 1990
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 18 (6), 517-522
- https://doi.org/10.1007/bf00327022
Abstract
A direct selection method was used to isolate centromeres from a genomic library of the yeast Kluyveromyces lactis. The method is based on the lethality at high copy number of the ochre-suppressing tRNA gene SUP11. Five different chromosomal fragments were found that confer mitotic stability to plasmids containing a replication origin of K. lactis (KARS). In addition, KARS plasmids containing these fragments have a copy number or approximately one, and each of the five fragments hybridizes to a different chromosome of K. lactis. From these results we conclude that five of the six centromeres of K. lactis have been isolated. These centromeres do not function in S. cerevisiae.Keywords
This publication has 28 references indexed in Scilit:
- A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1989
- Composite motifs and repeat symmetry in S. pombe centromeres: Direct analysis by integration of Notl restriction sitesCell, 1989
- Saccharomyces cerevisiae centromere CEN11 does not induce chromosome instability when integrated into the Aspergillus nidulans genome.Molecular and Cellular Biology, 1986
- Analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe.Proceedings of the National Academy of Sciences, 1986
- Orthogonal‐field‐alternation gel electrophoresis banding patterns of DNA from yeastsYeast, 1986
- Yeast centromeres: Structure and functionCell, 1984
- Structural Analysis and Sequence Organization of Yeast CentromeresPublished by Cold Spring Harbor Laboratory ,1983
- A high-frequency transformation system for the yeast Kluyveromyces lactisCurrent Genetics, 1982
- Isolation of a yeast centromere and construction of functional small circular chromosomesNature, 1980
- Analysis of gene control signals by DNA fusion and cloning in Escherichia coliJournal of Molecular Biology, 1980