Gas chromatographie determination of extracellular metabolites produced by baker’s yeast during glucose-induced acidification

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Abstract
Gas chromatographic separation of metabolites extruded during the medium acidification by resting baker’s yeast supplied with glucose is described. Silylation with bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane and a direct chromatography on Chromosorb G-AW-DMCS with 2.5 % SE-52 in a programmed temperature interval of 80–220 °C (3 °C/min) made it possible to separate organic acids (lactic, pyruvic, succinic, glyceric, fumaric, malic), polyols (glycerol, arabinitol) and sugars (glucose). The yeast was found to extrude glycerol, lactic, malic, and succinic acid.