Repertoires of T cells directed against a large protein antigen, beta-galactosidase. I. Helper cells have a more restricted specificity repertoire than proliferative cells.
- 1 April 1982
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 128 (4), 1529-1534
- https://doi.org/10.4049/jimmunol.128.4.1529
Abstract
The proliferative and helper T cell repertoires were compared in the CBA/J mouse for the response to the large protein antigen, tetrameric beta-galactosidase (GZ = 1021 a.a/monomer). The systems assessed the ability of cyanogen bromide (CB) peptides of GZ to: 1) prime for a T cell proliferative response to GZ; or 2) generate T cell help, measured by the production of anti-FITC PFC in the in vitro response to GZ-FITC. Priming for in vitro proliferation was attempted with 11 CB peptides comprising 70% of the GZ molecule. Strong priming was found with five peptides and intermediate priming was found with four other peptides; two peptides were without effect (CB-20 = a.a 767-862, and CB-4 = a.a. 188-202). Despite this indication of generally dispersed recognition of GZ epitopes, only two CB peptides, CB-2 (a.a. 3-92) and CB-10 (a.a. 378-418) were able to induce a T helper cell response. The surprising dearth of helper T cell-inducing epitopes may be peculiar to the limited fluorescein (FITC) substitution on GZ-FITC (17-25 FITC residues per tetramer) or it may reflect the constraints involved in T cell recognition required for T-B collaboration. Also considered was the possibility that the helper T cell repertoire might be distinct from the proliferative repertoire, the latter reflecting DNA synthesis and recruitment by other functional T cell subpopulations.This publication has 4 references indexed in Scilit:
- Epitope specificity of the T cell proliferative response to lysozyme: proliferative T cells react predominantly to different determinants from those recognized by B cellsEuropean Journal of Immunology, 1980
- Antigen‐specific stimulation and trans‐stimulation of T cells in long‐term cultureEuropean Journal of Immunology, 1979
- Amino acid sequence of beta-galactosidase. VII. Isolation of the 24 cyanogen bromide peptidesJournal of Biological Chemistry, 1978
- Key antigenic determinants in regulation of the immune response.Proceedings of the National Academy of Sciences, 1977