Simple standardized enzyme-linked immunosorbent assay for human antibodies to Entamoeba histolytica

Abstract

A simple solid-phase enzyme-linked immunosorbent assay [ELISA] procedure for the detection of human antibodies to E. histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis and indirect hemagglutination methods, as well as with clinical data. [ELISA] is rapid (1 h 15 min, total incubation time) and the reported values are referenced to a positive control so that they correlate with levels of antibody sufficient to be detected by the gel diffusion methods. ELISA is highly reproducible, specific and sensitive; it can be used qualitatively or quantitatively.