Correction for Inner Filter Effects in Fluorescence Spectroscopy

Abstract

A method is proposed for correcting experimental fluorescence readings for inner filter effects, i.e. the absorption of the exciting light and/or the absorption of the emitted radiation, which cause the non‐linearity between fluorescence intensity and fluorophor concentration. Basically the method consists of measuring the fluorescence intensity at two different points along the diagonal in the cell. Unlike similar methods proposed in the literature, the two points are corrected simultaneously for both absorption of excitation and of emission radiation without the necessity of reading the optical density of the solution, and with a very simple data elaboration.