Reovirus-induced modification of cap-dependent translation in infected L cells.
- 1 January 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (1), 152-156
- https://doi.org/10.1073/pnas.77.1.152
Abstract
The translational apparatus in cell-free extracts prepared from [mouse fibroblast] L cells infected with reovirus undergoes a time-dependent transition from cap dependence to cap independence. Extracts from uninfected L cells translate capped reovirus mRNA at high efficiency and synthesize the expected 3 size classes of reovirus polypeptides, and the translation is sensitive to m7G(5'')ppp. This same extract translates uncapped mRNA at a much lower efficiency. In contrast, extracts from infected L cells translate uncapped reovirus mRNA at high efficiency and synthesize the correct 3 size classes of polypeptides, and the translation is not sensitive to inhibition by m7G(5'')ppp. Infected cell extracts translate capped mRNA at reduced efficiency (.apprxeq. 25%), the translation is not sensitive to inhibition by m7G(5'')ppp, and the correct 3 size classes of viral polypeptides are not synthesized. These observations may explain how reovirus takes over the host translational apparatus.Keywords
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