Differences in Cellular Pathogenicity of Two Immunologically Related Poliomyelitis Viruses as Revealed in Tissue Culture.

Abstract

The Y-SK strain of poliomyelitis virus may be titrated in roller tube cultures by observation of the degeneration of monkey testicular fibroblasts within 4 days following the addition of virus to the tissue culture. The extent of fibroblastic degeneration is inversely related to the metabolic activity of the tissue, as measured by the amount of acid production. The 2 immunologically related Y-SK and Lansing strains of poliomyelitis virus differ in the degree to which they cause fibroblastic degeneration, and alter the amount of acid production by the tissue. In contrast to the Lansing strain, the Y-SK strain produces more extensive degeneration in fibroblasts of monkey testicles and also in those of human embryonic skin-muscle.