MARKER PROPERTIES OF TUMOR AND LYMPHOID-CELL LINES DERIVED FROM A PATIENT WITH SQUAMOUS-CELL CARCINOMA

Abstract

A squamous cell carcinoma tumor cell line, COLO 227, derived from a metastatic [skin] tumor in a Caucasian male, produced parathyroid hormone and carcinoembryonic antigen. A chromosome mode of 106 predominated and the X and Y chromosomes were retained. Seven marker chromosomes were identified. Cytogenetic analyses revealed an isochromosome 8 marker similar to a HeLa [cervical cancer] cell line marker and an isochromosome 17 marker described in other cancers. An autochthonous lymphoid cell line, COLO 219, was established and characterized. COLO 219 is a normal lymphoid cell line with B[bone marrow-derived]-cell characteristics. This autochthonous system of cultured tumor cells and cultured lymphocytes is of use in immunological studies.