Long-Term Culture of Tissue Cells in Magnet Stirred Fluid Suspensions.

Abstract

Human leukemic bone-marrow-derived (MCN) cells and human epidermoid carcinoma cells, (HeLa) and (H. Ep #1), have been continuously cultured over 1 year as suspended cell populations in a magnetstirred fluid medium. This method of cultivation is a practical means of providing large numbers of freely suspended tissue cells for immunochemical and other studies. Cell multiplication for all 3 strains studied is characterized by: a) an initial lag phase of 2–3 days, b) a phase of active cell multiplication, reaching maximum concentrations around 9–10 days, and c) a phase beginning around 12 days with no further increase in cell numbers. Under conditions described, maximum cell yields were obtained by collecting one-half the cells from populations in phase (b) each 3–4 days.