DETECTION AND EVALUATION OF FELINE ONCORNAVIRUS-INDUCED CELL-SURFACE ANTIGEN(S) SHED FROM CELLS INVITRO
- 1 January 1977
- journal article
- research article
- Vol. 37 (11), 4134-4137
Abstract
A method for preparation of soluble feline oncornavirus-induced cell surface antigens was described. This technique relied upon the natural release of antigen(s) from FL-74 feline lymphoblastoid cells during their maintenance at 37.degree. C in serum-deficient medium. When concentrated and clarified spent medium from 4 day cultures was tested for its antigen content by inhibition of humoral cytotoxicity, this natural production of soluble antigen provided more feline oncornavirus-associated cell membrane antigen per cell than did a solubilization procedure in which papain was used. The shed antigen preparation was immunogenic in cats, eliciting humoral antibody that was reactive with the surface of FL-74 cells and feline sarcoma virus-transformed nonproducer mink cells but was not reactive with feline leukemia virus in a virus neutralization assay.This publication has 10 references indexed in Scilit:
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