Expression of a human gene for interferon in yeast
- 1 October 1981
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 293 (5835), 717-722
- https://doi.org/10.1038/293717a0
Abstract
A DNA sequence coding for mature human leukocyte interferon D (LeIF-D) was linked with DNA fragments of the 5''-flanking sequences of the S. cerevisiae (yeast) alcohol dehydrogenase I gene in a plasmid capable of autonomous replication and selection in both yeast and Escherichia coli. Yeast cells transformed by these plasmids synthesize up to 1 .times. 106 molecules of biologically active LeIF-D/cell.Keywords
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