Purification of tetanus toxoid

Abstract

A simple method is described for the prepn. of purified and concentrated solns. of tetanus toxoid. Purified toxoids made from broths containing Martin''s peptone or neopeptone ranged in purity from 250 to 830 Lf/mg. X as compared with 3.3 Lf/mg. N to 5.0 Lf/mg. N for the original toxoids and 3300 Lf/mg. N as a probable value for pure toxoid. The method of purification did not rid the toxoid of Witte peptone when this constituent was present in the crude toxoid. Crude toxoids containing Martin''s peptone and neopeptone yield products in which these peptones could not be detected. The purified toxoids flocculated in less time than did the corresponding crude toxoids.