Apoprotein B of lipoprotein(a) of human plasma

Abstract

Lipoprotein(a) was purified by agarose-gel chromatography from human plasma from which lipoproteins of Sf [flotation coefficient] > 0 had been removed either by sequential or density-gradient ultracentrifugation. After delipidation, the apoprotein B of this lipoprotein was analyzed by sodium dodecyl sulfate/polyacrylamide-gel electrophoresis. It could not be distinguished from the apoprotein B of low-density lipoproteins (.rho. 1.019-1.063 g/ml). A significant increase in the concentration of apoprotein B in plasma from which the Sf > 0 lipoproteins had been removed was observed in 6 subjects 4 h after a fatty meal.